![]() Animal studies and recent clinical research suggests that B. All rights reserved.Banisteriopsis caapi is the basic ingredient of ayahuasca, a psychotropic plant tea used in the Amazon for ritual and medicinal purposes, and by interested individuals worldwide. Finally, they were applied to the analysis of a 145-bp sequence from the human gene MRP3, reaching a detection limit of 210 pg μL(-1).ĭNA sensing platform Diazonium chemistry Real DNA sample detection.Ĭopyright © 2014 Elsevier B.V. The hybridization event with the complementary sequence was detected using hexaamineruthenium (III) chloride as electrochemical indicator. The resulting DNA sensing platforms were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and spectroscopic ellipsometry. As a comparison a second strategy based on the binding of aminated DNA capture probes to the developed diazonized conducting platforms through a crosslinking agent was also employed. The presence of primary aromatic amines allows the formation of diazonium cations capable to react with the amines present at the DNA capture probe. The electrochemical reduction of 4-nitrobenzenediazonium salt onto screen-printed carbon electrodes (SPCE) in aqueous media gives rise to terminal grafted amino groups. Electronic address: simple strategy for covalent immobilizing DNA sequences, based on the formation of stable diazonized conducting platforms, is described. Electronic address: 8 Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain IMDEA Nanociencia, Madrid, Spain. Electronic address: 7 Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: 6 Department of Applied Physics, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: 5 Department of Applied Physics, Universidad Autónoma de Madrid, 28049 Madrid, Spain. ![]() Electronic address: 4 Department of Analytical and Food Chemistry, Universidad de Vigo, 36310 Vigo, Pontevedra, Spain. Electronic address: 3 Department of Analytical and Food Chemistry, Universidad de Vigo, 36310 Vigo, Pontevedra, Spain. Electronic address: 2 Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: 1 Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain. ![]() 1 Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
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